Journal: Sensors (Basel, Switzerland)

Article Title: Novel PCR Assays Complement Laser Biosensor-Based Method and Facilitate Listeria Species Detection from Food

doi: 10.3390/s150922672

Figure Lengend Snippet: Sensitivity of lap gene-based Listeria species sensu stricto -specific primer (ELAP-F1/LIS-R1) tested against L. monocytogenes F4244 and L. innocua F4248. Agarose gel showing amplifications with the primers for different concentrations of template DNA of L. monocytogenes and L. innocua in reaction volume of 25 µL. PCR products in the gel were quantified using the NIH ImageJ image processing and analysis software.

Article Snippet: Overnight (16 h) grown cells of L. monocytogenes F4244 (8.02 ± 0.11 log 10 CFU/mL) yielded 96.1 ± 2.1 ng/µL DNA, and 1 ng DNA was equivalent to 5.5 log 10 genomic equivalent (GE), whereas L. innocua F4248 (8.15 ± 0.24 log 10 CFU/mL) yielded 98.4 ± 0.6 ng/µL DNA, and 1 ng DNA was equivalent to 5.5 log 10 GE.

Techniques: Agarose Gel Electrophoresis, Software

Journal: Sensors (Basel, Switzerland)

Article Title: Novel PCR Assays Complement Laser Biosensor-Based Method and Facilitate Listeria Species Detection from Food

doi: 10.3390/s150922672

Figure Lengend Snippet: Positive predictive value (PPV, precision rate) for the scatter images of colonies obtained from thirteen L. monocytogenes serotypes grown on BHIA and LBA media.

Article Snippet: Overnight (16 h) grown cells of L. monocytogenes F4244 (8.02 ± 0.11 log 10 CFU/mL) yielded 96.1 ± 2.1 ng/µL DNA, and 1 ng DNA was equivalent to 5.5 log 10 genomic equivalent (GE), whereas L. innocua F4248 (8.15 ± 0.24 log 10 CFU/mL) yielded 98.4 ± 0.6 ng/µL DNA, and 1 ng DNA was equivalent to 5.5 log 10 GE.

Techniques: